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MICROSCOPY & CELL FRACTIONATION - ppt video online download
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Fractionation is a separation process in which a certain amount of mixture (gas, solid, liquid, enzyme, suspension, or isotope) is divided during the transition phase, into smaller amounts (fractions) where the composition varies according to gradient. Fractions are collected on the basis of differences in the specific properties of the individual components. A common feature in fractionation is the need to find the optimum between the number of fractions collected and the desired purity in each fraction. Fractionation allows to isolate more than two components in the mix in one run. This property distinguishes it from other separation techniques.

Fractionation is widely used in many branches of science and technology. The mixture of liquid and gas is separated by fractional distillation by the difference of the boiling point. Fractionation of components also occurs in column chromatography with differences in the affinity between the stationary phase and the mobile phase. In fractional crystallization and fractional fractions, chemicals are fractionated on the basis of differences in solubility at a certain temperature. In cell fractionation, the cell components are separated by mass differences.


Video Fractionation



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Bioassay-guided fractionation

In pharmacognism, a typical protocol for isolating pure chemical agents from natural origin is bioassay-guided fractionation, which means a step-by-step separation of components extracted by differences in their physicochemical properties, and assessing biological activity, followed by subsequent rounds of separation and testing.

Blood fractionation

The process of blood fractionation involves the separation of blood into its main component. Blood fractionation generally refers to the separation process using a centrifuge (centrifuge), after which the three major blood components can be visualized: plasma, buffy coat and erythrocytes (blood cells). These separate components can be analyzed and often separated further.

Food fractionation

Fractionation is also used for culinary purposes, such as coconut oil, palm oil, and cracked palm kernel oil to produce oils with different viscosities, which can be used for different purposes. This oil typically uses fractional crystallization (separation by solubility at temperature) for the separation process, not distillation. Mango oil is an oil fraction obtained during processing of mango butter.

Milk can also be fractionated to recover protein concentrates of milk or milk protein base fractions.

Maps Fractionation



Fractionation isotope


Isotope fractionation - YouTube
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Dose fractionation

The dose of radiotherapy is usually divided and transmitted for example. 5 days a week for a period of six weeks.

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See also

  • Copurification
  • Fractionated spaceship
  • List of chemical purification methods
  • Cipher Transposition # Fractionation

Cell Fractionation and Ultracentrifugation - www.examvault.net ...
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References

http://www.wyatt.com/products/hardware/eclipse-particle-sizing-systems-field-flow-fractionation.html

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Further reading

  • The Laboratory Handbook for Fractionation of Natural Extracts. , by Peter J. Houghtonand Amala Raman, publisher: Chapman & amp; Hall, 1998 - 199 pages

Source of the article : Wikipedia

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